Molecular analysis of sarcoidosis lymph nodes for microorganisms: a case–control study with clinical correlates

The 16S gene fragment was amplified as previously described.8 The hsp65 gene was amplified using TB11 and TB12 primers, and the RNA polymerase subunit gene (rpoB) was amplified using MF and MR primers.9 The amplified products were then sequenced using the Big Dye Sequencing kit (Applied Biosystems, Foster City, California, USA) as per the vendor's recommended protocol. The sequences of two strands were assembled into double-stranded contig using Sequenchersoftware (Gene Codes, Ann Arbor, Michigan, USA). The final sequences were used to search the National Center for Biotechnology Information (National Institutes of Health) database using the Basic Local Alignment Search Tool (BLAST) to identify the amplified DNA.

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