Complete Chloroplast Genome of Tanaecium tetragonolobum: The First Bignoniaceae Plastome

Contigs produced de novo were blasted against the original chloroplast genome reference in order to exclude contigs of nuclear origin. Contigs with coverage below 10x were eliminated, likely leading to the exclusion of contigs of mitochondrial origin as well. The remaining de novo and reference-guided contigs were assembled into larger contigs in Sequencher5.3.2 (Gene Codes Inc., Ann Arbor, MI) based on at least 20 bps overlap and 98% similarity.

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